A drone attack on a base in Jordan killed three United States troops on Sunday, with President Joe Biden blaming Iran-backed militants and vowing to hold the perpetrators to account.

It is the first time American military personnel have been killed by hostile fire in the Middle East since the start of the Israel-Hamas war, and the incident will further raise tensions in the region and fuel fears of a broader conflict directly involving Iran.

Hamas said the death of the soldiers shows Washington’s backing for Israel could put it at odds with the whole Muslim world if innocent lives continue to be lost in Gaza, and that the war there could lead to a “regional explosion”.

Biden, in a statement on the attack, said: “While we are still gathering the facts of this attack, we know it was carried out by radical Iran-backed militant groups operating in Syria and Iraq.

“We will carry on their commitment to fight terrorism. And have no doubt – we will hold all those responsible to account at a time and in a manner of our choosing.”

U.S. Central Command put the number of wounded from the attack near the Syrian border at 25 and said the identities of those killed will be withheld pending notification of their families.

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Hamas spokesman, Sami Abu Zuhri, said the killing of the soldiers “is a message to the American administration that unless the killing of innocent people in Gaza stops, it may be faced with the entire (Muslim) nation.”

“The continuation of the American-Zionist aggression on Gaza risks a regional explosion,” Abu Zuhri said in a statement.

U.S. and allied forces in Iraq and Syria have been targeted in more than 150 attacks since mid-October, according to the Pentagon, and Washington has carried out retaliatory strikes in both countries.

Many of the attacks on U.S. personnel have been claimed by the Islamic Resistance in Iraq, a loose alliance of Iran-linked armed groups that oppose U.S. support for Israel in the Gaza conflict.

On Sunday, the Islamic Resistance in Iraq said it targeted U.S. personnel with drones at three locations in Syria, including two bases near where the borders of Iraq, Syria, and Jordan meet, but it was not immediately clear if the group was referring to the attack that killed the American troops.

Meanwhile, Jordanian government spokesman, Muhannad Mubaidin, said the attack did not occur in Jordan and rather targeted a base in Syria.

The latest round of the Israel-Hamas conflict began when the Palestinian militant group carried out a shock attack on October 7 that resulted in about 1,140 deaths, mostly civilians, according to an AFP tally of official figures.

Following the attack, the United States rushed military aid to Israel, which has carried out a relentless military offensive that has killed at least 26,422 people in Gaza, most of them women and children, according to the Gaza health ministry.

Those deaths have sparked widespread anger across the region and stoked violence involving Iran-backed groups in Lebanon, Iraq, and Syria as well as Yemen.

The Star